ФІЗИКО-МЕХАНІЧНИЙ ІНСТИТУТ ІМ. Г.В. КАРПЕНКА НAH УКРАЇНИ
KARPENKO PHYSICO-MECHANICAL INSTITUTE OF THE NAS OF UKRAINE

 

Biotechnologies

1. Department of Optical-Digital Diagnostics Systems (# 5).

2. Subdirections:

  1. Research of biospeckle spatial distribution dynamics in biotissues of fruits and vegetables by methods and means of spatial-temporal speckle correlation.
  2. Study of cellular activity of muscular tissues of domestic animals and poultry for development of technologies for nondestructive testing of meat food freshness level by digital speckle correlation techniques. 
  3. Development of new methods and systems of digital speckle pattern interferometry and digital speckle correlation for estimation of fruits, vegetables and other vegetation agricultural production quality during their harvesting, storage, freezing and processing.

3. Projects: acting, finished and perspective

Finished projects

            1. Ukrainian – Polish joint research project „Study of change dynamics of spatial distribution of fruit biospeckle-pattern” that implemented in frameworks of Protocol to the Agreement on scientific cooperation between the Polish Academy of Sciences (PAS) and the National academy of sciences of Ukraine (2009-2011 years).  Executors: Karpenko Physico-Mechanical Institute of the NAS of Ukraine, Project Leader – Head of Dept. of optical-digital diagnostics systems (# 5), DrSci, Prof. L.I. Muravsky; Bohdan Dobrzański Institute of Agrophysics of PAS, Project Leader – Vice Director of Institute Prof. Artur Zdunek.

2. Agreement about scientific and technical collaboration between State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives and Karpenko Physico-Mechanical Institute of the NAS of Ukraine from 12.09.2014 to 11.09. 2016 years. Agreement was concluded with the purpose to promote the development of joint scientific researches, adoption of rmodern and creation of new optical-electronic methods for study veterinary medicinal products and  animal feed production, joint participation in scientific events, publication of joint scientific researches results.

Perspective Projects

The call for proposals of the joint Ukraine-Poland R&D Projects for the period of 2017 – 2018, announced by the Ministry of Education and Science of Ukraine and the Ministry of Science and High Education of Poland:  “Detection of subsurface defects and physiologic disorders in fruits and vegetables with the use of active thermography and digital speckle pattern interferometry”. Participants: Karpenko Physico-Mechanical Institute of the NAS of Ukraine, Project Leader – Head of Dept. of optical-digital diagnostics systems (# 5), DrSci, Prof. L.I. Muravsky; Bohdan Dobrzański Institute of Agrophysics of PAS, Project Leader – Head of Department of Metrology and Modelling of Agrophysical Processes, Prof. IA PAS, DrSci Piotr Baranowski

4. Designs and technologies (techniques).

            1. Technique for processing of biospeckle patterns to establish connection between changes of spatial distributions of correlation coefficient values and biochemical processes in fruits and vegetables.           

2. Spatial-temporal speckle correlation technique for operational assessment of structural changes in muscle tissues of meat feed after slaughtering.

5. Publications

1. Франкевич Л.Ф., Максименко О.П., Муравський Л.І. Дослідження біоспеклів методом просторово-часової спекл-кореляції // Відбір і обробка інформ. – 2005. – Вип. 23 (99). – С. 117-121.

2. Frankevych L.F., Maksymenko O.P., Muravsky L.I. Study of biospeckle pattern moverment speed with the spatial-temporal digital speckle correlation technique // Review of Current Problems in Agrophysics / Ed. by G. Józefaciuk, C. Sławiński, R.T. Walczak – Lublin, 2005. – P. 339–340.

3. Muravsky L.I., Maksymenko O.P., Frankevych L.F. Studing of botanical specimen ageing with spatial-temporal speckle correlation technique // 3rd Intern. Scientif. Conf. “Influence of Electromagnetic Field on Agricultural Environment “AGROLASER 2006”, Lublin 5-7.09.2006. – Lublin, 2006. – P. 83–89.

4. New nondestructive method based on spatial-temporal speckle correlation technique for evaluation of apples quality during shelf-life / A. Zdunek, L.I. Muravsky, L. Frankevych, K. Konstankiewicz // International Agrophysics. – 2007. – 21, N 3. – P. 305–310.

5. Zdunek A., Frankevych L., Konstankiewicz K., Ranachowski Z. Comparison of puncture test, acoustic emission and spatial-temporal speckle correlation technique as methods for apple quality evaluation // Acta Agrophysica. – 2008. – 11, N 1. – P. 303–315.

6. Muravsky L., Frankevych L. Monitoring of apple quality during shelf-life storage by the spatial-temporal speckle correlation technique // Intern. Conf. “Physics in Agrocultural Research”, June 12-13, Lublin, Poland. Papers and short communications. – Lublin, 2008. – P. 98.

7. Франкевич Л., Муравський Л., Адамяк А. Дослідження пошкоджень поверхні біологічних об’єктів методом просторово-часової спекл-кореляції // Фізичні методи в екології, біології та медицині: Матер. IV Міжнар. конф. (Львів-Шацьк, 15-18 вересня 2011 р.). – Львів, 2011. – С. 110-111.

8. Використання динамічних спекл-полів для дослідження процесів in vitro у м’язовій тканині / О.П. Максименко, І.Я. Коцюмбас, В.І. Ткаченко, М.І. Березюк, Л.І. Муравський, О.М. Щебентовська // Відбір і обробка інформації. – 2013. – Вип. 38 (114). –С. 61–68.

9. Березюк М.І., Максименко О.П., Ткаченко В.І., Санагурський Д.І. Деякі аспекти дослідження структури м’язової тканини in vitro методом просторовочасової кореляції спеклів // Біологічні Студії. – 2013. – 7, №1. – С. 55–62.

10. Application of biospeckles for assessment process in sceletal muscles / O. Maksymenko, L. Muravsky, M. Berezyuk, V. Tkachenko // 10th International Conference on Agrophysics, 5th- 7 th June, 2013, Lublin, Poland. Book of abstracts. P. 52.

11. Laser speckle application for assessment process in skeletal muscles / O. Maksymenko, L. Muravsky, M. Berezyuk, V. Tkachenko // Laser technologies. Lasers and their application: Materials of International Scientific & Technical Conference. Drohobych. – 2013.– P. 127–128.

12. Maksymenko O.P., Muravsky L.I., Berezyuk M.I. Application of biospeckles for assessment of structural and cellular changes in muscle tissue // J. Biomed. Opt. – 2015. – 20, N 4. – P.095006-1-7.

6. The problem state.

Improvement of agricultural production quality is one of prominent direction promoting the development of the agrarian sector of economy and agro-food systems. This direction is inseparably linked with solving of problems for evaluation of characteristics of farm products that include fruits, vegetables and meat foods to define optimal harvesting, preserving and processing conditions and terms. There is a number of biotechnological methods, namely sensory evaluation, which gives only qualitative results, and various mechanical tests (punch, pressure tests and so on), which are characterized by destruction during study process, and thus are undoubtedly imperfect. On the other hand, nondestructive optical-digital techniques based on analysis of biospeckle patterns by using spatial-temporal speckle correlation have developed successfully. Our department as one of the first among research groups has started to deal with a problem of use of spatial-temporal speckle correlation techniques to study the cellular activity of biotissues (see Refs. in Section 5).  In particular, mechanisms of temporal changes of biospeckle spatial distributions displaying cellular activity of fruit and vegetable tissues by using spatial-temporal speckle correlation technique were established. Studying of these mechanisms allows elaborating the methods for estimation of apples, tomatos and other fruit and vegetable freshness and shelf life (see Refs. ). Besides, researches of meat food for operational assessment of structural changes in muscle tissues after slaughtering by using optical-digital correlation methods were also fulfilled . Now we start to develop new techniques based on digital speckle pattern interferometry for detection and localization of different defects and physiologic disorders in fruits and vegetables during their harvesting, storage and processing. This direction is also very challenging, because a lot of data about cellular processes and their spatial-temporal disturbances in studied biological objects is accumulated in temporal series of biospeckle fringe patterns.